Insertion junction: CLIP2.018176_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018176
Chromosome:chromosome 9
Location:2558902
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392652 (1 of 1) 1.5.1.22 - Strombine dehydrogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGACGCACCCACCACCCCCGCACGCACCGCACGCACCGCACGCCGCCG
Internal bar code:CAGTAAGCACAAGAGCACATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGCCCAATCATCCACAA
Suggested primer 2:CACCCGGGTATCATGTACGG