| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018204 |
| Chromosome: | chromosome 11 |
| Location: | 1492400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTTGGCGTAGGGTGCGGCCAGTGGGAGGAGCTTGTGGCTACGCTAG |
| Internal bar code: | TAGGGAAATCATGAGACGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1986 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATTGGTTGCGATGTAC |
| Suggested primer 2: | GCTTAGAGCAATGGAACGCG |