| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018215 |
| Chromosome: | chromosome 7 |
| Location: | 5492199 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351150 | FA2 | (1 of 1) PTHR24362//PTHR24362:SF229 - SERINE/THREONINE-PROTEIN KINASE NEK // SEVEN TRANSMEMBRANE DOMAIN-CONTAINING SERINE/THREONINE-PROTEIN KINASE 2; Flagella Abscission 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCCGAATCACGTGTGGGTGATTCAGCTGTGCAAGCATGCGAGCCTCA |
| Internal bar code: | GGGGCATGTCAGCACGTAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2216 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAAGCCCGGGACAGTTG |
| Suggested primer 2: | GCATTCGTACATCACCACGC |