Insertion junction: CLIP2.018216_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.018216
Chromosome:chromosome 3
Location:3932173
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g171100 (1 of 77) IPR001623 - DnaJ domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCACGTCTCCGCCCTCCATCCCAACCCTCATCCCCACCTGGGTTT
Internal bar code:ACTGTTTATGAACATTGATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1102
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCTAAAGATGCGCCTCCG
Suggested primer 2:GTGATCATGGTGATTGGCGC