| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018218 |
| Chromosome: | chromosome 9 |
| Location: | 6097101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412880 | HSP70F | Heat shock protein 70F; (1 of 2) PTHR19375:SF173 - CHAPERONE PROTEIN DNAK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGGGCCGCCCGGGGAGTGCCGTACTTGTGTGCGTGTTGTTGTCGGT |
| Internal bar code: | CGTTGATAGAATGGTTTACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGTATGATCCGCCAGGG |
| Suggested primer 2: | ACGAGCTCCTCAAAGGTGTG |