Insertion junction: CLIP2.018223_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.018223
Chromosome:chromosome 2
Location:6042943
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118801 GTR Glycosyl transferase; (1 of 1) 2.4.1.246 - Mannosylfructose-phosphate synthase / MFPS intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCACCTGCCCACCTGTCGACGTTGCCACGCACACGCGTATGCACGCA
Internal bar code:GTGTGGGCGTGGGTGATCTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1318
LEAP-Seq percent confirming:70.3704
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGACGGACCATGTTTGAG
Suggested primer 2:CAGAACAGCACCAACCAACG