Insertion junction: CLIP2.018242_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.018242
Chromosome:chromosome 6
Location:2396263
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268550 GTR,CGL121,CSL1 Cellulose-synthase-like 1; (1 of 1) 2.4.1.32 - Glucomannan 4-beta-mannosyltransferase / Glucomannan-synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTATGTGACTTAGCTAAGGTGGGCCTGCTCTCGGCCCTATAGCGGCTT
Internal bar code:AATTCGGTCTGCCTCTAATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1224
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCACCCACACACCATTT
Suggested primer 2:CCCAGTGTGTGCCACCTATT