Insertion junction: CLIP2.018242_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.018242
Chromosome:chromosome 6
Location:2396310
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268550 GTR,CGL121,CSL1 Cellulose-synthase-like 1; (1 of 1) 2.4.1.32 - Glucomannan 4-beta-mannosyltransferase / Glucomannan-synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAGCGACTGCGTCCCGTGATCCCACGTCCCAAGTGCCCACTACTGTG
Internal bar code:CCCAATGTCCCATTATAATAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:45.4545
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGTGTGTGCCACCTATT
Suggested primer 2:AACCCACCCACACACCATTT