| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.018256 |
| Chromosome: | chromosome 7 |
| Location: | 5882069 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353850 | (1 of 1) K15289 - solute carrier family 35, member F5 (SLC35F5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCACAGCCTCCTTCCTTGCATCAGACCCTAACGAAGCGCCGCTTCCC |
| Internal bar code: | GTGGCAGAGACTGGAGTTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1494 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATCATCGGCGGCTTCAT |
| Suggested primer 2: | ATACCGTTCTGCCCACAGTG |