Insertion junction: CLIP2.018286_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018286
Chromosome:chromosome 9
Location:153329
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386732 (1 of 1) 2.4.1.57 - Phosphatidylinositol alpha-mannosyltransferase / Phosphatidyl-myo-inositol alpha-mannosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCACCTGCAGTACAAGCCGTCACACGCACCCTCCCCCACCAAAACCC
Internal bar code:GCTTGTAGATCGGATTTCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1440
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCAACTCCACTGTGACG
Suggested primer 2:ACCGCCTTGTTGTCGTACTT