| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018319 |
| Chromosome: | chromosome 7 |
| Location: | 3953669 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339633 | DNJ20 | (1 of 2) PTHR24078//PTHR24078:SF257 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGATCCCGGCTTGCCGGGCCCTGCTGGTTCTGTTCCCTGCAGGTGCCG |
| Internal bar code: | GCCGGTGAATCTCATGGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4705 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCTCTCGTGGAGGACCT |
| Suggested primer 2: | ATGAGTGAAACGGCGTGCTA |