| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018366 |
| Chromosome: | chromosome 6 |
| Location: | 3294859 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277350 | HDA13 | RPD3/HDA1 type histone deacetylase; (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTCATCCACCCGAGACACGCCCGCGCACCTCGATAGTGTGTTGAACT |
| Internal bar code: | TGATAGGTGTCGTATGTCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGGACAGGTTGAAGGG |
| Suggested primer 2: | CACCTCAAGCCGTACACACT |