Insertion junction: CLIP2.018396_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018396
Chromosome:chromosome 8
Location:2256911
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g370601 (1 of 1) K14559 - U3 small nucleolar RNA-associated protein MPP10 (MPP10) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATGGGGATGGCGATGATGAGGACGGCGGCGCGGAGCGGCGGCGCTCG
Internal bar code:TAATGATATGTATATATTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1679
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAAGGGGGTTGCAACACA
Suggested primer 2:CGCCTTCCTTGCGCATTATC