| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.018415 |
| Chromosome: | chromosome 6 |
| Location: | 5842180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289350 | PRF2,PRFA2 | (1 of 2) PTHR11075:SF9 - PEPTIDE CHAIN RELEASE FACTOR 1, MITOCHONDRIAL-RELATED; Prokaryotic-type peptide release factor A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGCTGATCGACTGCTACGGGCAGTAGCGGTGGCATAGCCGGGGCCC |
| Internal bar code: | CTCTTGAGCCCATCAAAATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2124 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAAGCGCGTACATGCAG |
| Suggested primer 2: | CAGTACAGTCGGTGGCAGTT |