Insertion junction: CLIP2.018427_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018427
Chromosome:chromosome 2
Location:2739628
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800206 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGGCCGAACATTCCGTTGCCGAATGACATTCAGGTGGGCGCTGAGGA
Internal bar code:GTTGTGCATCATTTTTGCGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3791
LEAP-Seq percent confirming:88.5057
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGCTGTGGTACTCGTTCA
Suggested primer 2:GGACGATCGCTCTAGTGTGG