| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018431 |
| Chromosome: | chromosome 10 |
| Location: | 5241523 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456250 | TRX16,TRX25,TRL11 | (1 of 4) PTHR10438//PTHR10438:SF255 - THIOREDOXIN // THIOREDOXIN M4, CHLOROPLASTIC; Thioredoxin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTCGGGGAAAAAGTCAGCAATACACAACGATGCCGTGCATGCGGCTC |
| Internal bar code: | TTTGGCATGGTATATGACAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 90.566 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAGGCAGCTATCTCAG |
| Suggested primer 2: | CAAACAACCGACAAGCCCAG |