| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018434 |
| Chromosome: | chromosome 10 |
| Location: | 612186 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421700 | GPDH1,GPD4 | Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase; (1 of 4) 1.1.1.8 - Glycerol-3-phosphate dehydrogenase (NAD(+)) / NADH-dihydroxyacetone phosphate reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACGGTGAGGGAGGGGGAAGCAGCGGCGGGGCGGGGCGGGACGGGG |
| Internal bar code: | CATAAGGTCTGTTTGTTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 78.7879 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTTGGAGCTCAAGTACT |
| Suggested primer 2: | GGAGAGGGTTAGGGGATGGT |