Insertion junction: CLIP2.018446_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018446
Chromosome:chromosome 3
Location:560539
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145267 (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATAATGCTGGGCGGAGCCGTGCCGTGTGATGTTTGCCACCCCCAACT
Internal bar code:GTATATAAAGGTCTCATTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3338
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGCACACTCGTCTTCAT
Suggested primer 2:ACACACATCCAGTCGCACAT