Insertion junction: CLIP2.018446_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018446
Chromosome:chromosome 3
Location:560907
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145267 (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGGGAGAAGCGTGGGTGGGTGAGCGTGATTAATAGATGCAGAAGCAG
Internal bar code:GTATATAAAGGTCTCATTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:555
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACATCCAGTCGCACAT
Suggested primer 2:ACACGCACACTCGTCTTCAT