| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.018448 |
| Chromosome: | chromosome 12 |
| Location: | 2220140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508852 | (1 of 1) 6.3.4.10 - Biotin--[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase / Propionyl-CoA holocarboxylase synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGAGGGCGCCGGGTATCGCAGCGCCAGGACGGCCTTGGAGTCGGCGC |
| Internal bar code: | GTCTTGCCTGTGAGTTGTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2051 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTTTTGGGGATGGGAG |
| Suggested primer 2: | ATTTTGAGCTAGCCGGCACT |