Insertion junction: CLIP2.018451_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018451
Chromosome:chromosome 2
Location:5450790
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800237 (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGTGGAACCCTCGCTCTCGCGCACGCTTCTTTCCTGCCCGCTTCTGA
Internal bar code:GCTGGTATTTTCTTATATTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1310
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCTTGCTAGACTCGATC
Suggested primer 2:CTGGATGACTCAGAGCGCAT