| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018456 |
| Chromosome: | chromosome 16 |
| Location: | 5359680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683450 | AGP2 | (1 of 4) 2.7.7.27 - Glucose-1-phosphate adenylyltransferase / ADP-glucose synthase; ADP-glucose pyrophosphorylase large subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCGGAACTCATCCGGGAGGGTGTCAAGGTGCGTGCAGCACACCCAC |
| Internal bar code: | ATTCGTTGAGGTCTCGCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1387 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAGGCGAGTTGATGGGG |
| Suggested primer 2: | CCGTCGACCAGTATCTCAGC |