Insertion junction: CLIP2.018465_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.018465
Chromosome:chromosome 12
Location:5781079
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g532750 EEP8 (1 of 1) K10772 - AP endonuclease 2 (APEX2); Endonuclease/exonuclease/phosphatase family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGCGGTCGTGCCTGCCGCCACAGCCATGCCTGATGCTACTGCCGGTC
Internal bar code:TTGGATGAAGGTATTCATGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1673
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TAACCATGTTCATGCGGCCT
Suggested primer 2:CCGCAAACAACATCTGCACA