Insertion junction: CLIP2.018470_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018470
Chromosome:chromosome 16
Location:30432
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g696050 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCATCTCGTTCGGGGCGCAGGAGCATGCTGCCACGTCGCCTCATGCC
Internal bar code:CGTAAATCCTGGGGAGATTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2735
LEAP-Seq percent confirming:98.9362
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:CATGATGAGCCATGTGCACG
Suggested primer 2:CGAATTCGAGACGGAGGAGG