| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.018471 |
| Chromosome: | chromosome 12 |
| Location: | 4180449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517750 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTTGCTTGCGGTACTGAGGACACCGGGGCATTGCAAATGGCGTGTGG |
| Internal bar code: | ACTTAGCCGATAAACGCTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1600 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACTTGACAGGTTGGCTG |
| Suggested primer 2: | CCGCCAGGCCAGAGTTATAG |