| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018478 |
| Chromosome: | chromosome 8 |
| Location: | 4005471 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382590 | (1 of 1) K11662 - actin-related protein 6 (ACTR6, ARP6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGCACGCATGTCGCATGCTGGAGCGTCCTGGGGCATTTCCCCAGCCA |
| Internal bar code: | TATAGGAACACATAGTGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1635 |
| LEAP-Seq percent confirming: | 79.6875 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCAGGCTCAAACAAACG |
| Suggested primer 2: | GGGAGGGAGGGGATTACAGT |