Insertion junction: CLIP2.018479_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018479
Chromosome:chromosome 11
Location:3054060
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g474750 (1 of 1) 2.4.2.39 - Xyloglucan 6-xylosyltransferase / Xyloglucan 6-alpha-D-xylosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCGGACTCCACAGGTGGGCAGGCAGGGTGCCCGGTTTTAGGGGTCGC
Internal bar code:TGGTTCGTGGAGTATAGGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:5529
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:108

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTGAACGCACATTGTGGC
Suggested primer 2:AGTAAGGTGAAGTGGCGTGG