Insertion junction: CLIP2.018488_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.018488
Chromosome:chromosome 17
Location:6515347
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g744697 (1 of 726) IPR011009 - Protein kinase-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGAACAGAGCCACGGCCTCCTGCAAACCCGCCAGCTCCTCTGCTGC
Internal bar code:GACGTTACATGGGGCACTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1177
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGTCGTGACGATCTGAG
Suggested primer 2:CACTCCATCTCCACCAGCAG