Insertion junction: CLIP2.018501_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018501
Chromosome:chromosome 7
Location:1985228
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325759 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTACTCTCAAGCTCTTCCTGTTCTTCCTGTTGTCAAAAATTGTTTTCT
Internal bar code:GTATTTAAGTAATCATAGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:338
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGGGGTTAGGGATTGGG
Suggested primer 2:CCTTCCACACAAAGCACGTG