Insertion junction: CLIP2.018524_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.018524
Chromosome:chromosome 3
Location:3877709
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g170550 POC4 Proteome of centriole protein 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGTATTACCATTCGATCATTACAGTGACTGGGCTGACCAGCATGGCA
Internal bar code:TCCGTAAGACCAGTTTATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2122
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAGCAGGCGACTTACCGA
Suggested primer 2:TGAGCTGAGAAAGAAGCGCA