Insertion junction: CLIP2.018562_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018562
Chromosome:chromosome 12
Location:5902837
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533600 DESI6,DeSI-6 (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAGCCACGCACAATCTGGTGCGAGTGCACCCATGTGATTTGCGGCGT
Internal bar code:TTATGCAGCAGCCCGTTTTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1842
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTGCTTTGAGTGTACGT
Suggested primer 2:GAACTGTGGCCCTGAATGGA