Insertion junction: CLIP2.018587_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018587
Chromosome:chromosome 12
Location:9204417
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g543050 (1 of 1) PF04241 - Protein of unknown function (DUF423) (DUF423) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGCGCTCGGTGACGCTTTGTCACGGCCCTGCCCCTCGCGCCCCTTGC
Internal bar code:TCAGTACGTCTAATGGGTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2908
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAGGTCCCCAGAATTTC
Suggested primer 2:CCGCATGCACTTGATTCGAG