Insertion junction: CLIP2.018598_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.018598
Chromosome:chromosome 7
Location:2319546
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTTGGACTTCTGGTGGTCCTATCAACGGCAAAACGCGCCTAATCAAC
Internal bar code:TATTTGTGGGGACATAGATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1865
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGCAGGTTAGCAGGAA
Suggested primer 2:GTGTTATCATGGGCGCGTTC