| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018605 |
| Chromosome: | chromosome 2 |
| Location: | 1217266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081700 | PHC66 | Pherophorin-chlamydomonas homolog 66; (1 of 1) IPR003882//IPR005404//IPR024616 - Pistil-specific extensin-like protein // Potassium channel, voltage dependent, Kv3.3 // Pherophorin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGGGAGGCTCCGGCGACGGCGGAGGGGCACCGTACACGGGCGGTGG |
| Internal bar code: | GAAGGCCCGTCCAGCGCAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1561 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGGTTCGCTTGTATGCA |
| Suggested primer 2: | ATTCCCATTCCCATTGCCGT |