Insertion junction: CLIP2.018606_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.018606
Chromosome:chromosome 9
Location:2861458
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390750 ELG24 (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 24 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGGGGGGGGGGCGGCGGCGGGTAGGAAGGCGGGAGGGAGGGTTACGT
Internal bar code:TATAATTCACGTGGTACTCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:244
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACAAGGACGGCAAGAAG
Suggested primer 2:ACGACACCCCTCACACAATC