Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.018615 |
Chromosome: | chromosome 1 |
Location: | 5149618 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g035950 | (1 of 1) K03321 - sulfate permease, SulP family (TC.SULP) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCCGTCACCTGCGGCGGCAAGCGCGGTTCCACGTTCGGTATTTAGC |
Internal bar code: | TGACTCGTTCGACATTAGTCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 330 |
LEAP-Seq percent confirming: | 85.1852 |
LEAP-Seq n confirming: | 23 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACAACACCTGCTGTCTGC |
Suggested primer 2: | GTCCGCACTTCCATCCTTGA |