Insertion junction: CLIP2.018615_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018615
Chromosome:chromosome 1
Location:5149620
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g035950 (1 of 1) K03321 - sulfate permease, SulP family (TC.SULP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACGCGGCGGCGGTGGCTCTGGGCTCGGCGCTGTGTGTCATGACCCTC
Internal bar code:TGACTCGTTCGACATTAGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:528
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGCACTTCCATCCTTGA
Suggested primer 2:CAACAACACCTGCTGTCTGC