Insertion junction: CLIP2.018656_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.018656
Chromosome:chromosome 8
Location:4423169
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g385350 (1 of 5) PTHR32467//PTHR32467:SF15 - FAMILY NOT NAMED // AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCGAGCGCTCGGGGCTCACCAGGTACACGTGCTTGCCAGAACTGTTG
Internal bar code:GGATCCTTCCAAAGGTAACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2700
LEAP-Seq percent confirming:96.9697
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGTCGCATGCATCTGG
Suggested primer 2:CGGGTACTGCTGCTGGTAAT