Insertion junction: CLIP2.018657_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018657
Chromosome:chromosome 6
Location:1643637
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261950 ANK11 (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats 5'UTR
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAAGTGTGATAGCTCCCCTGTACGTGCATTAATGTAGCAGGATTGGTT
Internal bar code:GGGAAATGTGCGTCCGGGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1626
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTACTGCATTCTCGCTGC
Suggested primer 2:ACGAGTGTGTGTGTGTGTGT