| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.018674 |
| Chromosome: | chromosome 7 |
| Location: | 1269878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322000 | CSD2,NIFS2 | (1 of 1) PTHR11601//PTHR11601:SF20 - CYSTEINE DESULFURYLASE // SELENOCYSTEINE LYASE; Cysteine desulfurase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACTGCTGGGGCTGAGGCTGGGGCTGGGGCTGGTAGCGCCGCCGCCG |
| Internal bar code: | TTGCTTATCTGTATTAAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1334 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAACTCACAACCTCCCTC |
| Suggested primer 2: | CGTGTGTGTGTTTGAGCCTG |