Insertion junction: CLIP2.018707_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018707
Chromosome:chromosome 2
Location:5981474
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118350 MFT14 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACCGCGTCTGCGGCAGCAGCTTCAGCAGCTGCGGCAGCTGTTTCAGG
Internal bar code:CGATTATAGCATTTGCTTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3663
LEAP-Seq percent confirming:98.4848
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CATGAGCCTTACTTCCCGCA
Suggested primer 2:GATGAACGAGGAGGAGAGCG