| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.018708 |
| Chromosome: | chromosome 16 |
| Location: | 7315755 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678773 | BSD2,BHSD2 | (1 of 1) PTHR10366//PTHR10366:SF355 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // SUBFAMILY NOT NAMED; 3-beta hydroxysteroid dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCTAGTCCAAATCCTCATGCTGGCGGAGCGCGGCCTGCGGACGCCGT |
| Internal bar code: | GTTGCTGCTTATTGGCGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3429 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCGATCCGGAACCAGTG |
| Suggested primer 2: | TATGGCTTGGATGCGGTGTT |