| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.018712 |
| Chromosome: | chromosome 3 |
| Location: | 5054498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179961 | POLE1,POLE1-1 | (1 of 1) K02324 - DNA polymerase epsilon subunit 1 (POLE1); DNA polymerase epsilon subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACCCACTTCATTGGGCTCGGGCACACAACACCCCTTCCCCCCCAC |
| Internal bar code: | ACATTCAGTTCTAGAGAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1424 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACATCGACACGGTGAGGG |
| Suggested primer 2: | GGTGGAGTGTTCAGTCCAGG |