Insertion junction: CLIP2.018714_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.018714
Chromosome:chromosome 6
Location:7595297
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g301806 (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAGTGGCGTCACTTACCGGGTGACATCCACAGGAGCTGCAGGGAGAAG
Internal bar code:ATCGGCTGAACCCACGTCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1033
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGACGAAGACGCACTGC
Suggested primer 2:AGGAGCAAACGACACCTGAG