Insertion junction: CLIP2.018790_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.018790
Chromosome:chromosome 8
Location:4023011
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g800955 (1 of 1) PF00400//PF13191 - WD domain, G-beta repeat (WD40) // AAA ATPase domain (AAA_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACGTCATAAGCAGGTCGGCACGCACGCAGTCATACACGCACTCACA
Internal bar code:TGAGGTTAACGTATAGCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTCTCCCAATCCCTTT
Suggested primer 2:AAACCAACGAAAACGAGCGG