| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.018792 |
| Chromosome: | chromosome 6 |
| Location: | 5486554 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286300 | UMM5,COQ5B | (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTTGTAGTACTGCGGGTACAGCTTGCCGCCGTCCAGCCACACGGGCT |
| Internal bar code: | AACTGGAGATTCTAGACGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5347 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGTGTGGTTGCCTTTG |
| Suggested primer 2: | CATGCATGGACCTGATTGCG |