| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018805 |
| Chromosome: | chromosome 12 |
| Location: | 5080002 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525650 | SUFS,SUFS1,CSD4 | (1 of 1) K11717 - cysteine desulfurase / selenocysteine lyase (sufS); Group II cysteine desulfurase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGATTGATGTGTGCGCACTTGGACCCGCAGGAGATTGGCACCTACCT |
| Internal bar code: | TTTGCGGTACATGTTTCTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3112 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTGCTCATCCACTCCT |
| Suggested primer 2: | TACGAGCTGCTGGAGGAGAT |