| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018833 |
| Chromosome: | chromosome 6 |
| Location: | 1679051 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262350 | (1 of 1) IPR003722 - Cobalamin (vitamin B12) biosynthesis CobH/CbiC, precorrin-8X methylmutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGCCAGAAGGGACCTTGGGACATCATGCCGAAGGTTGCTCTGCGGT |
| Internal bar code: | GAGGTAGTTCGTTTGAGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1946 |
| LEAP-Seq percent confirming: | 83.0189 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCCGTGTTACGCAATAT |
| Suggested primer 2: | AGCATGATAGACGCCTGCTC |