Insertion junction: CLIP2.018876_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018876
Chromosome:chromosome 5
Location:3145551
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g800573 (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGTGTTTGCGGCGGCGACGCCTGTTCTGGCGCCGCGCTGGGCGCTGC
Internal bar code:ACTTATGATACTGATTTGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1482
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTACCAGTCTGCCAGCA
Suggested primer 2:GATGAGTAGTGGTGGTGGCG