| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018914 |
| Chromosome: | chromosome 12 |
| Location: | 1350470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489200 | (1 of 1) PTHR10794//PTHR10794:SF28 - ABHYDROLASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGGCAACCTTCACCGTCGCCTTGGCGGTTGCCACCGACCCGGGCCC |
| Internal bar code: | ATTGCCATGGAATGTATGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6031 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGTGTCCGGTAGCTAC |
| Suggested primer 2: | CAGACTGAACCTGCCTCTGG |